Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Stem cell factor binding to retrovirus primer binding site silencers

M Yamauchi1, B Freitag, C Khan

  • 1Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland 97201.

Journal of Virology
|February 1, 1995
PubMed
Summary

A novel embryonal carcinoma cell factor binds to a stem cell-specific silencer, regulating Moloney murine leukemia virus (M-MuLV) expression. This binding correlates with M-MuLV silencer activity and may impact retroviral replication in stem cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Self-reported financial difficulties of colorectal cancer patients 1 year after start of treatment.

ESMO open·2025
Same author

[How to write a good clinical review?].

Nuklearmedizin. Nuclear medicine·2015
Same author

[Implementation of the S3 prostate cancer guideline in daily clinical practice: results of a survey among urologists].

Der Urologe. Ausg. A·2014
Same author

HRTEM of 1DSnTe@SWNT nanocomposite located on thin layers of graphite.

Journal of microscopy·2012
Same author

Using a monochromator to improve the resolution in TEM to below 0.5 Å. Part II: application to focal series reconstruction.

Ultramicroscopy·2012
Same author

The structure of nanocomposite 1D cationic conductor crystal@SWNT.

Journal of microscopy·2012

Area of Science:

  • Molecular Biology
  • Virology
  • Stem Cell Biology

Background:

  • Stem cell-specific silencers regulate gene expression at the DNA level.
  • Moloney murine leukemia virus (M-MuLV) proline primer binding site (PBS) is involved in viral replication.
  • Embryonal carcinoma (EC) cells provide a model for studying stem cell differentiation.

Purpose of the Study:

  • To investigate the binding of an EC cell factor (binding factor A) to a stem cell-specific silencer.
  • To determine the relationship between factor A binding and M-MuLV silencer activity in vivo and in vitro.
  • To explore the role of different PBS DNA fragments in M-MuLV repression and factor binding.

Main Methods:

  • Modified nuclear lysis and binding assays to study factor A binding to the M-MuLV silencer.

Related Experiment Videos

  • In vivo repression assays using various PBS DNA fragments.
  • In vitro DNA binding assays to assess factor-DNA interactions.
  • Partial purification of binding factor A.

    • Main Results:

    • In vitro binding of factor A correlated with in vivo M-MuLV silencer activity.
    • Factor A bound specifically to the wild-type silencer element at optimal temperatures and preferred the full-length element over the core.
    • Factor A was enriched in nuclear extracts and undifferentiated EC cells, and partially purified as a >100 kDa heparin-Sepharose-binding moiety.
    • The lysine-1,2 PBS DNA fragment showed high repression of M-MuLV and bound a related EC cell factor, though it did not cross-compete with factor A.

    Conclusions:

    • Binding factor A plays a role in stem cell-specific M-MuLV silencing.
    • The lysine-1,2 PBS is crucial for M-MuLV repression in stem cells.
    • These findings suggest potential inhibition of certain retroviruses (Mason-Pfizer monkey virus, visna virus, spumavirus) in undifferentiated stem cells due to related factor interactions.