Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evidence for a second cell cycle block at G2/M by p53

N Stewart1, G G Hicks, F Paraskevas

  • 1Manitoba Institute of Cell Biology, Manitoba Cancer Treatment and Research Foundation, Winnipeg, Canada.

Oncogene
|January 5, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome Wide Conditional Mouse Knockout Resources.

Drug discovery today. Disease models·2024
Same author

Genetically programmed retinoic acid deficiency during gastrulation phenocopies most known developmental defects due to acute prenatal alcohol exposure in FASD.

Frontiers in cell and developmental biology·2023
Same author

Clinical presentation, sonographic features and treatment options of segmental stiff skin syndrome.

Clinical and experimental dermatology·2020
Same author

Skin lesions in a diabetic patient.

The Netherlands journal of medicine·2019
Same author

Part of the union.

Nursing standard (Royal College of Nursing (Great Britain) : 1987)·2016
Same author

Modulating effects of WT1 on interferon-β-vitamin D association in MS.

Acta neurologica Scandinavica·2014

Wild type p53 induces cell cycle arrest. Mutant p53, when co-expressed with H-ras, also causes cell cycle arrest at G1/S and G2/M, suggesting p53

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • Wild type p53 protein is known to arrest the cell cycle at G1/S.
  • Most p53 mutants lose this cell cycle arrest ability.
  • Previous studies showed p53 mutants can rescue cells from ras-induced arrest, while wild type p53 inhibits growth.

Purpose of the Study:

  • To investigate if p53 can induce cell cycle arrest at the G2/M boundary.
  • To analyze the role of p53 in cell cycle regulation at different checkpoints.

Main Methods:

  • Utilized the REF52 cell line.
  • Employed a temperature-sensitive p53val135 mutant allele.
  • Enriched cells in late G1/early S phases before temperature shift.
  • Analyzed cell cycle progression using flow cytometry.

Related Experiment Videos

Main Results:

  • REF52 cells expressing mutant p53val135 and activated H-ras arrested at both G1/S and G2/M phases at the restrictive temperature.
  • Flow cytometry confirmed these cell cycle arrest points.

Conclusions:

  • p53's anti-proliferative activity is involved in regulating the G2/M checkpoint.
  • p53 also plays a role in G1/S transit and DNA synthesis initiation.
  • These findings highlight p53's multifaceted role in cell cycle control.