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[Physicochemical studies of Pharmachem's tylosin]
Veterinarno-Meditsinski Nauki
|January 1, 1978
Summary
Tylosine exhibits excellent thermal stability but degrades under light. Protect tylosine solutions from light and store in dry conditions to maintain antibiotic activity and chemical stability.
Area of Science:
- Pharmaceutical Chemistry
- Microbiology
Background:
- Tylosine is an important antibiotic with various salt forms.
- Understanding its stability is crucial for effective pharmaceutical formulations.
Purpose of the Study:
- To evaluate the chemical stability and biological activity of tylosine base, tylosine tartarate, and tylosine phosphate.
- To determine the impact of environmental factors like temperature, light, moisture, and solvents on tylosine.
Main Methods:
- Spectrophotometric analysis in the UV-region.
- Microbiological assays using agar diffusion with Sarcina lutea.
- Assessment of tylosine under varying temperature, light, humidity, and solvent conditions.
Main Results:
- Tylosine is thermally stable but sensitive to UV and direct sunlight, leading to significant activity loss.
- Tylosine salts exhibit varying degrees of hygroscopicity, absorbing substantial moisture at high humidity.
- Certain solvents like dimethylformamide and dimethylsulphoxide do not affect activity but inhibit diffusion assays.
Conclusions:
- Tylosine solutions require protection from light during preparation, storage, and application.
- Proper storage in dry, airy conditions or hermetic packaging is essential to prevent moisture absorption.
- Careful selection of solvents is necessary for accurate biological activity assessments.