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Updated: Jul 15, 2026

Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
Published on: June 12, 2013
Hydrostatic pressure effects upon cellular leakage and active transport by Vibrio marinus
Abstract:
Hydrostatic pressures greater than ca. 400 atm cause release of cellular components of Vibrio marinus MP-1 in the order protein greater than RNA greater than malate dehydrogenase greater than DNA greater than amino acids. Increased pressures also slow the rate of cycloleucine (non-metabolizable analogue of L-leucine acid L-valine) uptake but increase its efflux rate. Lineweaver-Burk plots (1/V versus 1/[S]) of cycloleucine uptake indicate that the Km increases with compression which suggests a decrease in affinity of the transport system for substrate at increased pressures.
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