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Aminoacyl-RNA synthesis catalyzed by an RNA
M Illangasekare1, G Sanchez, T Nickles
1Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347.
Science (New York, N.Y.)
|February 3, 1995
Abstract:
An RNA has been selected that rapidly aminoacylates its 2'(3') terminus when provided with phenylalanyl-adenosine monophosphate. That is, the RNA accelerates the same aminoacyl group transfer catalyzed by protein aminoacyl-transfer RNA synthetases. The best characterized RNA reaction requires both Mg2+ and Ca2+. These results confirm a necessary prediction of the RNA world hypothesis and represent efficient RNA reaction (> or = 10(5) times accelerated) at a carbonyl carbon, exemplifying a little explored type of RNA catalysis.