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Relationship between nuclear morphology and DNA content in large cell lymphomas
J Billiet1, A Criel, J van Vuchelen
1Department of Hematology and Pathology, Algemeen Ziekenhuis Sint-Jan, Brugge, Belgium.
Analytical and Quantitative Cytology and Histology
|October 1, 1994
Summary
Large cell lymphomas (LCLs) show distinct nuclear morphology linked to DNA content. DNA tetraploid LCLs have larger, irregular nuclei compared to DNA diploid LCLs, aiding ploidy prediction.
Area of Science:
- Hematopathology
- Cell Biology
- Cytometry
Background:
- Large cell lymphomas (LCLs) exhibit variable nuclear morphology.
- Understanding the factors influencing nuclear appearance is crucial for diagnosis.
Purpose of the Study:
- To investigate the relationship between DNA content and nuclear morphology in LCLs.
- To determine if nuclear features can predict DNA ploidy levels in LCLs.
Main Methods:
- Microscopic evaluation of nuclear size, shape, and chromatin pattern on May-Grünwald-Giemsa stained lymph node imprints.
- Image analysis of DNA content and nuclear area on Feulgen-stained slides.
- Correlation analysis between nuclear morphology parameters and DNA ploidy.
Main Results:
- Twelve of 24 LCL cases were DNA diploid, and 12 were DNA tetraploid.
- DNA tetraploid cases showed significantly larger nuclei (mean MND 13.0-19.1 microns) and more coarse chromatin and nuclear irregularities than diploid cases (mean MND 11.2-13.7 microns).
- Morphological assessment predicted ploidy in 80% of cases, with a strong correlation (0.84) between mean nuclear diameter and nuclear area.
Conclusions:
- DNA content, reflecting ploidy and cell cycle stage, significantly influences nuclear morphology in LCLs.
- Nuclear morphology provides valuable clues for predicting DNA ploidy in LCLs.
- Nuclear size increases as cells progress through the cell cycle.