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Updated: Oct 12, 2026

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes
Published on: August 13, 2012
Regulation of CFTR channel gating
1Laboratory of Cardiac/Membrane Physiology, Rockefeller University, New York, NY 10021.
Abstract:
The debilitating symptoms of cystic fibrosis stem from the reduced Cl- permeability of epithelial cells owing to mutations in the gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel. In cells with normal CFTR channels, receptor-mediated activation of cyclic-AMP-dependent protein kinase causes phosphorylation of several serines in the regulatory domain of CFTR, permitting channel opening and closing via cycles involving ATP hydrolysis. Cellular phosphatases rapidly dephosphorylate the channels, inactivating them. Here we discuss recent advances in our understanding of this complex mechanism for regulating channel gating.
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