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Detection of proteins and sialoglycoproteins in polyacrylamide gels using eosin Y stain
1Department of Anatomy and Cell Biology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803.
Molecular Biotechnology
|April 1, 1994
Abstract:
An eosin Y staining technique that permits detection of various proteins, including membrane sialoglycoproteins, in polyacrylamide gels is described. The sensitivity of the eosin Y staining method is comparable to silver staining. In addition, there is an added advantage of the antigenicity of the stained proteins being retained in a Western blot. Details of the procedure to obtain optimal staining results are described.