Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The dopamine transporter: immunochemical characterization and localization in brain

B J Ciliax1, C Heilman, L L Demchyshyn

  • 1Emory University School of Medicine, Department of Neurology, Atlanta, Georgia 30322.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|March 1, 1995
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An interim exploratory proteomics biomarker analysis of a phase 2 clinical trial to assess the impact of CT1812 in Alzheimer's disease.

Neurobiology of disease·2024
Same author

Complex spatial and temporally defined myelin and axonal degeneration in Huntington disease.

NeuroImage. Clinical·2018
Same author

In vitro antioxidant/prooxidant effects of combined use of flavonoids.

Natural product research·2017
Same author

Poorly understood and often miscategorized congenital umbilical cord hernia: an alternative repair method.

Hernia : the journal of hernias and abdominal wall surgery·2016
Same author

Alpha-2 macroglobulin in Alzheimer's disease: a marker of neuronal injury through the RCAN1 pathway.

Molecular psychiatry·2016
Same author

Cough-Associated Changes in CSF Flow in Chiari I Malformation Evaluated by Real-Time MRI.

AJNR. American journal of neuroradiology·2015

Researchers developed specific antibodies for the dopamine transporter (DAT) to map its location in the brain. This tool aids in understanding dopamine pathways and related disorders.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Immunology

Background:

  • The dopamine transporter (DAT) plays a crucial role in regulating dopaminergic neurotransmission.
  • Understanding DAT's distribution is key to comprehending dopamine system function and dysfunction in neurological and psychiatric disorders.

Purpose of the Study:

  • To develop and characterize novel antibodies specific for the dopamine transporter (DAT).
  • To utilize these antibodies for the immunolocalization of DAT protein in the rat brain.
  • To investigate the regional and cellular distribution of DAT.

Main Methods:

  • Generation of antibodies targeting different regions of the DAT protein (N-terminus, extracellular loop, C-terminus).
  • Characterization using immunoblot analysis, immunoprecipitation, and immunocytochemistry.

Related Experiment Videos

  • Validation through preabsorption studies and lesioning of dopaminergic pathways.

    • Main Results:

    • Developed and validated DAT-specific antibodies that recognize DAT in rat and human tissues.
    • Immunocytochemistry revealed DAT distribution in key brain regions, largely consistent with dopaminergic innervation.
    • Observed discrepancies between DAT and tyrosine hydroxylase distribution suggest heterogeneity in dopaminergic systems.

    Conclusions:

    • The generated DAT antibodies are specific and effective tools for studying DAT localization.
    • Findings highlight the heterogeneity of dopaminergic systems and potential independent regulatory mechanisms.
    • These antibodies will facilitate further research into DAT's role in neurological and psychiatric conditions.