[Assessment of blood lymphocyte subpopulations by the immunoenzyme cytochemical PAP method using different fixatives]

The method of fixation of cytologic material was modified for a relatively safe immunocytochemical staining with para-aminophenasine (PAPh) in order to provide the intactness of the principal differentiation antigens of lymphocytic surface membranes of human peripheral blood. The method is recommended for practical studies of immunophenotypic lymphocyte subpopulations in HIV-infected subjects and patients with AIDS and other infectious diseases.