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Efficiency of MHC class I antigen processing: a quantitative analysis

M S Villanueva1, P Fischer, K Feen

  • 1Section of Infectious Diseases, Yale School of Medicine, New Haven, Connecticut 06520-8022.

Immunity
|September 1, 1994
PubMed
Summary
This summary is machine-generated.

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Listeria monocytogenes antigen processing efficiency was studied. Approximately 35 p60 molecules degrade to yield one cytotoxic T lymphocyte epitope, revealing insights into the MHC class I pathway.

Area of Science:

  • Immunology
  • Microbiology
  • Molecular Biology

Background:

  • Listeria monocytogenes is an intracellular pathogen.
  • p60 is a secreted murein hydrolase processed into a T cell epitope.
  • The H-2Kd MHC class I molecule presents the p60 epitope to T cells.

Purpose of the Study:

  • To quantify the efficiency of antigen processing for the L. monocytogenes p60 protein.
  • To investigate the relationship between intracellular antigen quantity and epitope production.
  • To understand the capacity of the MHC class I antigen processing pathway.

Main Methods:

  • Utilized L. monocytogenes strains with varying p60 secretion levels.
  • Monitored the production and degradation rates of p60 and its epitope.

Related Experiment Videos

  • Calculated antigen processing efficiency based on secretion and degradation kinetics.

    • Main Results:

    • p60 217-225 epitope production rate is directly proportional to intracellular antigen load.
    • Epitope generation is linked to the degradation of newly synthesized p60.
    • Estimated that ~35 p60 molecules degrade to produce one p60 217-225 epitope.

    Conclusions:

    • Provides an estimate for the efficiency of antigen processing in the MHC class I pathway.
    • Suggests the MHC class I pathway can accommodate foreign antigens.
    • Highlights the quantitative aspects of intracellular antigen processing for T cell recognition.