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NADPH-diaphorase localization in the olfactory system

H Zhao1, S Firestein, C A Greer

  • 1Section of Neurobiology and Neurosurgery, Yale University School of Medicine, New Haven, CT 06510, USA.

Neuroreport
|December 30, 1994
PubMed
Summary
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NADPH-diaphorase staining reveals its role in the olfactory system. Its presence changes with nerve transection, suggesting involvement in NO and CO signaling for developing and adult olfactory functions.

Area of Science:

  • Neuroscience
  • Olfactory System Biology
  • Biochemistry

Background:

  • NADPH-diaphorase (NADPHd) is an enzyme present in the nervous system.
  • Its role in the olfactory system, particularly concerning nitric oxide (NO) and carbon monoxide (CO) signaling, requires further elucidation.
  • Olfactory system development and adult function involve complex molecular pathways.

Purpose of the Study:

  • To investigate the distribution and changes in NADPH-diaphorase staining within the olfactory system.
  • To explore the potential involvement of NADPHd in NO and CO modulation in the olfactory epithelium and bulb.
  • To differentiate the role of NADPHd in developing versus adult olfactory systems.

Main Methods:

  • NADPH-diaphorase histochemical staining was performed on intact olfactory systems of rats and salamanders.

Related Experiment Videos

  • Olfactory nerve transections (salamander) and bulbectomies (rat) were conducted to study deafferentation effects.
  • Staining patterns were analyzed in the olfactory epithelia, nerve, and glomerular layers of the olfactory bulb.

    • Main Results:

    • Intense NADPHd staining was observed in the olfactory epithelia (mucosal surface) and olfactory bulb (nerve and glomerular layers) of intact animals.
    • Transection or bulbectomy led to decreased staining at the mucosal surface but increased staining in deeper epithelial cells.
    • Deafferentation resulted in the loss of staining in the olfactory nerve and glomerular layers of the bulb.

    Conclusions:

    • NADPH-diaphorase plays a significant role in the olfactory system.
    • NADPHd may serve as a substrate for NO biosynthesis, modulating cGMP in the developing olfactory epithelium.
    • A complementary role for CO signaling involving NADPHd is suggested in the adult olfactory system.