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Oligoclonal CD8+ T cells are preferentially expanded in the CD57+ subset
J K Morley1, F M Batliwalla, R Hingorani
1Department of Medicine, North Shore University Hospital, Manhasset, NY 11030, USA.
Journal of Immunology (Baltimore, Md. : 1950)
|June 1, 1995
Summary
Oligoclonal expansion, a feature of CD8+ T cells, is common in the CD57+ subset of normal individuals. This accumulation in CD57+CD8+ T cells suggests a role in immune homeostasis.
Area of Science:
- Immunology
- T cell biology
- Immune system homeostasis
Background:
- Oligoclonality and clonal expansion are common in CD8+ T cells.
- Elevated CD57+CD8+ T cells are noted in certain disease states.
Purpose of the Study:
- To compare the T cell receptor (TCR) repertoire of CD57+ and CD57- subsets of CD8+ T cells in healthy individuals.
- To investigate the prevalence and characteristics of T cell populations within these subsets.
Main Methods:
- Utilized three-color Fluorescence-Activated Cell Sorting (FACS) analysis.
- Employed a panel of 16 monoclonal antibodies (mAbs) specific for TCR V segments.
- Analyzed T cell receptor repertoires in CD57+ and CD57- CD8+ T cell subsets from 41 normal volunteers.
Main Results:
- The CD57 surface marker was present on a minority (mean 16.3%) of CD8+CD3+ T cells.
- Dramatic oligoclonal expansion was observed in 37% of the CD57+CD8+ T cell populations.
- Prominent oligoclonal expansions were not observed in the CD57-CD8+ subset, though some oligoclonality was present at lower frequencies.
Conclusions:
- Oligoclonal expansion is a characteristic feature of the normal immune system, particularly within the CD57+CD8+ T cell population.
- The accumulation of oligoclonal CD8+CD57+ T cells suggests a role in immune homeostasis.
- The precise function and reasons for this accumulation remain to be elucidated.