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Related Experiment Videos

Multi-enzyme reference material from established human cell lines and human sources

Y Marui1, C Hayashi, Y Matsuda

  • 1Central Laboratory of Clinical Investigation, Saiseikai Nakatsu Hospital, Osaka, Japan.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|January 16, 1995
PubMed
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A new multi-enzyme reference material, stable and human serum-like, aids clinical chemistry standardization. It shows high accuracy for six enzymes but requires further development for alkaline phosphatase standardization.

Area of Science:

  • Clinical Chemistry
  • Biochemistry
  • Enzymology

Background:

  • Accurate enzyme activity measurement is crucial for clinical diagnostics.
  • Standardization of enzyme assays requires reliable reference materials.
  • Human serum-based calibrators can be variable and difficult to obtain.

Purpose of the Study:

  • To develop and evaluate a multi-enzyme reference material for clinical chemistry.
  • To assess the stability and performance of the reference material.
  • To compare its utility against human serum and commercial controls for enzyme standardization.

Main Methods:

  • Purification of seven human enzymes (AST, ALT, ALP, LD, CK, gamma-GT, AMY) from cell lines.
  • Preparation of a lyophilized multi-enzyme reference material.

Related Experiment Videos

  • Intermethod comparison of enzyme activities in clinical specimens using different calibrators.

    • Main Results:

    • The multi-enzyme material exhibited enzymatic properties similar to human serum.
    • The lyophilized preparation was stable for at least 200 days at 40°C.
    • The reference material provided comparable results to human serum for six enzymes (AST, ALT, LD, CK, gamma-GT, AMY).
    • Results for alkaline phosphatase (ALP) were not satisfactory, indicating a need for further optimization.

    Conclusions:

    • The developed multi-enzyme reference material shows promise for standardizing enzyme assays in clinical chemistry.
    • It offers a stable and reproducible alternative to human serum calibrators for most tested enzymes.
    • Further research is needed to identify a suitable reference material for alkaline phosphatase standardization.