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Real-time Live Imaging of T-cell Signaling Complex Formation
Published on: June 23, 2013
Phosphotyrosine-dependent association between CD22 and protein tyrosine phosphatase 1C
1Department of Immunology, Scripps Research Institute, La Jolla, CA 92037, USA.
Abstract:
CD22 is a B lymphocyte-specific cell surface glycoprotein that becomes tyrosine phosphorylated upon B cell activation. To determine if tyrosine phosphorylated CD22 couples signaling through membrane immunoglobulin (mIg) to down-stream elements, we looked for molecules coprecipitating with CD22 after anti-Ig stimulation. We found that a 60-kDa molecule was stably associated with CD22 following cross-linking of mIg and have identified this molecule as protein tyrosine phosphatase 1C (PTP1C). The association between PTP1C and CD22 is dependent upon tyrosine phosphorylation of CD22, but does not appear to require tyrosine phosphorylation of PTP1C.
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