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CD44 in differentiated embryonic stem cells: surface expression and transcripts encoding multiple variants

H Haegel1, A Dierich, R Ceredig

  • 1INSERM U184, CNRS LGME, Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France.

Developmental Immunology
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

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Embryonic stem cells (ES cells) initially lack surface CD44 but develop it during differentiation, suggesting a role in early mouse development.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Background:

  • CD44 is a cell-surface adhesion molecule implicated in various biological processes.
  • Understanding CD44 expression during early embryonic development is crucial for deciphering cell differentiation pathways.

Purpose of the Study:

  • To investigate the expression patterns of CD44 during in vitro differentiation of mouse embryonic stem cells (ES cells).
  • To identify the specific CD44 transcripts and variants expressed during ES cell differentiation.

Main Methods:

  • Immunofluorescence analysis to detect surface CD44 expression.
  • Northern blotting to analyze CD44 mRNA transcripts.
  • Polymerase Chain Reaction (PCR) to identify specific CD44 variable exons.

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Main Results:

  • Undifferentiated ES cells showed no surface CD44 but expressed 1.6 and 3.3 kb transcripts.
  • Differentiated ES cells exhibited surface CD44 expression and a 4.5 kb mRNA species.
  • At least three distinct CD44 variant transcripts, including exon v10 and sometimes v6, were induced during differentiation.

Conclusions:

  • CD44 expression is dynamically regulated during ES cell differentiation.
  • The induction of specific CD44 variants suggests a role in cell adhesion and migration during early mouse embryogenesis.