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[Yersinia pestis superoxide dismutase]

O A Kulikov, V I Drobkov, I V Darmov

    Vestnik Rossiiskoi Akademii Meditsinskikh Nauk
    |January 1, 1995
    PubMed
    Summary

    Yersinia pestis contains two superoxide dismutases. High molecular-weight superoxide dismutase from Y. pestis offers protection against plague, suggesting its potential as a vaccine component.

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    Area of Science:

    • Microbiology
    • Immunology
    • Enzymology

    Background:

    • Superoxide dismutases (SODs) are critical enzymes for cellular defense against oxidative stress.
    • Yersinia pestis, the causative agent of plague, possesses SODs that may play a role in its virulence and survival.
    • Understanding Y. pestis SODs is important for developing effective countermeasures against plague.

    Purpose of the Study:

    • To identify and characterize superoxide dismutases in Yersinia pestis EV cells.
    • To investigate the properties and regulation of Y. pestis superoxide dismutases.
    • To evaluate the immunogenicity and protective efficacy of Y. pestis superoxide dismutase.

    Main Methods:

    • Enzyme identification and molecular weight determination.
    • Analysis of antigenic specificity, thermal resistance, and inhibitor sensitivity.
    • Investigation of thermoinducible activity and release into culture medium.
    • Humoral response assessment in patients and immunization studies in guinea pigs.

    Main Results:

    • Two superoxide dismutases (65-67 kD and 200 kD) were identified in Y. pestis EV cells.
    • These enzymes exhibited differences in antigenic specificity, heat resistance, and inhibitor susceptibility.
    • Superoxide dismutase activity was thermoinducible and not plasmid-dependent, with enzymes released into the medium.
    • A humoral response to high molecular-weight SOD was observed in patients vaccinated with live plague vaccine.
    • Immunization of guinea pigs with purified high molecular-weight SOD conferred specific protection against plague.

    Conclusions:

    • Yersinia pestis possesses distinct superoxide dismutases with unique characteristics.
    • High molecular-weight superoxide dismutase from Y. pestis is immunogenic and provides protective immunity.
    • This high molecular-weight superoxide dismutase is a promising candidate for plague vaccine development.

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