Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Porcine E-selectin: cloning and functional characterization

Y T Tsang1, P E Stephens, S T Licence

  • 1Celltech Research, Celltech Ltd, Slough, Berkshire, UK.

Immunology
|May 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bariatric surgery and pain outcomes in osteoarthritis.

Osteoarthritis and cartilage·2021
Same author

A low balance between microparticles expressing tissue factor pathway inhibitor and tissue factor is associated with thrombosis in Behçet's Syndrome.

Scientific reports·2016
Same author

The exaggerated inflammatory response in Behçet's syndrome: identification of dysfunctional post-transcriptional regulation of the IFN-γ/CXCL10 IP-10 pathway.

Clinical and experimental immunology·2015
Same author

Methotrexate-mediated activation of an AMPK-CREB-dependent pathway: a novel mechanism for vascular protection in chronic systemic inflammation.

Annals of the rheumatic diseases·2015
Same author

Pegylated interferon-α-2b reduces corticosteroid requirement in patients with Behçet's disease with upregulation of circulating regulatory T cells and reduction of Th17.

Annals of the rheumatic diseases·2014
Same author

Skin stem cell hypotheses and long term clone survival--explored using agent-based modelling.

Scientific reports·2013

Researchers characterized porcine E-selectin, crucial for leukocyte-endothelium adhesion during inflammation. They found it supports neutrophil adhesion and identified its specific leukocyte recognition patterns, offering insights into inflammatory processes.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • E-selectin mediates initial leukocyte-endothelial adhesion in inflammation.
  • Understanding porcine E-selectin is vital for comparative immunology and inflammatory research.

Purpose of the Study:

  • To characterize the porcine E-selectin molecule.
  • To investigate its functional role in leukocyte adhesion.
  • To compare its structure and function to human E-selectin.

Main Methods:

  • Monoclonal antibody cross-reactivity studies.
  • cDNA isolation and sequence homology analysis.
  • Functional adhesion assays with neutrophils.
  • Expression studies on activated endothelial cells.
  • Chimeric molecule binding studies.

Related Experiment Videos

Main Results:

  • Porcine E-selectin is a 92,000 MW glycoprotein recognized by anti-human E-selectin antibody.
  • Porcine E-selectin cDNA shows 71% homology to human E-selectin but with structural differences.
  • It supports adhesion of both porcine and human neutrophils.
  • Expression on activated porcine endothelial cells is sustained for up to 48 hours.
  • Binding studies indicate recognition of granulocytic leukocytes and a lymphocyte subpopulation.

Conclusions:

  • Porcine E-selectin shares functional and structural similarities with human E-selectin.
  • It plays a significant role in leukocyte adhesion to the endothelium in pigs.
  • This molecule recognizes specific leukocyte subsets, contributing to inflammatory cell recruitment.