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TABS, a T cell activation antigen that induces LFA-1-dependent aggregation
1Department of Pathology, Stanford University, CA 94305, USA.
Journal of Immunology (Baltimore, Md. : 1950)
|August 15, 1995
Summary
A new antibody, DATK44, identifies TABS, a molecule involved in T cell activation and lymphocyte aggregation. TABS expression changes during T cell development and is re-expressed upon activation, mediating aggregation via the LFA-1 pathway.
Area of Science:
- Immunology
- Cell Biology
Background:
- Monoclonal antibody DATK44 recognizes a 50 kDa glycoprotein, termed TABS (T cell activation B cell subset Ag).
- TABS is expressed on monocytes, neutrophils, lymphocytes, and high endothelial venules, with differential regulation during T lymphocyte development.
Purpose of the Study:
- To characterize the TABS antigen and its role in lymphocyte aggregation.
- To investigate the signaling pathways involved in DATK44-induced homotypic aggregation.
Main Methods:
- Monoclonal antibody production and characterization.
- Flow cytometry for TABS expression analysis during T cell development and activation.
- Cell aggregation assays with TK1 lymphoma cells.
Main Results:
- TABS expression is dynamic, appearing on activated T lymphocytes and B cells, and differentially regulated in thymocytes.
- DATK44-induced TK1 cell aggregation is temperature-sensitive and dependent on metabolic activity.
- Aggregation is mediated by the LFA-1 pathway, as evidenced by blockade with anti-LFA-1 and anti-ICAM-1 antibodies.
Conclusions:
- TABS is a novel adhesion inducer that selectively activates LFA-1-mediated lymphocyte aggregation.
- Understanding TABS function may provide insights into immune cell trafficking and T cell activation processes.