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Related Experiment Videos

Cross-linking cell surface class II molecules stimulates Ig-mediated B cell antigen processing

A E Faassen1, S K Pierce

  • 1Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|August 15, 1995
PubMed
Summary

Cross-linking B cell class II molecules enhances antigen processing and presentation initiated by surface immunoglobulin binding. This signaling pathway selectively augments antigen processing, highlighting a key regulatory mechanism in B cell function.

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Area of Science:

  • Immunology
  • Cellular Signaling
  • Antigen Presentation

Background:

  • T helper (Th) cells interact with peptide-MHC class II complexes on B cells.
  • Cross-linking of MHC class II molecules on B cells can signal and modulate B cell functions.
  • Class II signaling may regulate the assembly of processed antigen-MHC class II complexes.

Purpose of the Study:

  • To investigate if cross-linking B cell surface class II molecules affects antigen processing and presentation.
  • To determine the specificity of class II-mediated signaling on antigen processing pathways.

Main Methods:

  • Cross-linking of B cell surface class II molecules.
  • Assessing antigen processing and presentation to T cell hybrids.
  • Evaluating antigen uptake via surface immunoglobulin versus fluid phase pinocytosis.

Related Experiment Videos

  • Measuring Ig internalization, MHC class II biosynthesis, and costimulatory molecule expression.

    • Main Results:

    • Cross-linking class II molecules augmented the processing and presentation of an antigen restricted by Ek.
    • This augmentation was specific to antigen processing initiated by surface immunoglobulin binding.
    • Antigen processing via pinocytosis or presentation of pre-processed peptides was unaffected.
    • Dibutyryl cAMP treatment enhanced the augmentation of antigen processing.

    Conclusions:

    • Cross-linking of B cell class II molecules selectively stimulates immunoglobulin-mediated antigen processing.
    • A specific step within the Ig-mediated antigen processing pathway is a target of class II-mediated signaling.
    • Class II signaling plays a regulatory role in B cell antigen processing and presentation.