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Related Experiment Videos

Cellular interactions in tissue factor expression by blood monocytes

B Osterud1

  • 1Department of Biochemistry, University of Tromsø, Norway.

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
|June 1, 1995
PubMed
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Cellular interactions, particularly involving granulocytes, platelets, and monocytes, are crucial for high tissue factor (TF) activity in response to lipopolysaccharide (LPS). This highlights the role of cell-cell communication in triggering blood coagulation.

Area of Science:

  • Hematology
  • Immunology
  • Cell Biology

Background:

  • Tissue factor (TF) is the primary initiator of the extrinsic pathway of blood coagulation.
  • TF is expressed on extravascular cells, forming a protective barrier that activates coagulation upon vascular injury.
  • Under pathological conditions, monocytes can express TF, potentially leading to aberrant blood coagulation.

Purpose of the Study:

  • To investigate the mechanisms underlying the high induction of TF activity in monocytes from certain individuals (high responders) upon stimulation with lipopolysaccharide (LPS).
  • To elucidate the cellular interactions and molecular mediators involved in this heightened TF response.

Main Methods:

  • Monocyte-based assays in whole blood to measure TF activity.
  • Investigating the role of P-selectin-mediated cell adhesion between granulocytes, platelets, and monocytes.

Related Experiment Videos

  • Assessing the contribution of platelet factor 4 (PF4) in TF induction.

    • Main Results:

    • Observed a significantly higher TF activity in monocytes of 'high responders' stimulated with LPS.
    • Demonstrated that this phenomenon is dependent on P-selectin-mediated interactions between granulocytes, platelets, and monocytes.
    • Identified the release of platelet factor 4 (PF4) as a key factor in mediating this cellular interaction and subsequent TF expression.

    Conclusions:

    • Cellular interactions play a central role in the expression of TF activity in circulating monocytes.
    • The heightened TF response in high responders is a complex process involving specific cell-cell communications and mediators like PF4.
    • Understanding these mechanisms is crucial for comprehending pathological coagulation activation.