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Related Experiment Videos

Multiple isoforms of guinea pig decay-accelerating factor (DAF) generated by alternative splicing

M Nonaka1, T Miwa, N Okada

  • 1Department of Molecular Biology, Nagoya City University School of Medicine, Japan.

Journal of Immunology (Baltimore, Md. : 1950)
|September 15, 1995
PubMed
Summary

Researchers identified guinea pig decay-accelerating factor (DAF, CD55) cDNA clones, revealing variable C-terminal regions and multiple isoforms. This suggests the Ser/Thr-rich region

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Decay-accelerating factor (DAF, CD55) regulates complement activation, preventing self-cell damage.
  • DAF is a GPI-anchored glycoprotein found only in primates at the molecular level.

Purpose of the Study:

  • To isolate and characterize guinea pig DAF cDNA clones.
  • To investigate the structural variability and isoforms of guinea pig DAF.

Main Methods:

  • Isolation of guinea pig DAF cDNA clones from a spleen library.
  • Sequence analysis to identify repeat domains and C-terminal variations.
  • Analysis of alternative splicing and splice site usage.

Main Results:

  • Six classes of guinea pig DAF cDNA clones were identified, sharing four short consensus repeat domains with human DAF.

Related Experiment Videos

  • Variability in the C-terminal region, generated by alternative splicing of optional exons, results in transmembrane, GPI-anchored, and secreted forms.
  • Differential splice site usage in a quintuplicated exon creates diverse Ser/Thr-rich regions, with isoforms ubiquitously expressed.

    • Conclusions:

    • Guinea pig DAF exhibits structural diversity through alternative splicing, similar to human membrane cofactor protein (MCP).
    • The variability in the Ser/Thr-rich region is crucial for effective regulation of complement activation.
    • Independent sequence multiplication in DAF and MCP highlights the functional importance of this region in RCA regulation.