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Platelet-derived microvesicles and activated platelets express factor Xa activity

P A Holme¹, F Brosstad, N O Solum

¹Research Institute for Internal Medicine, Rikshospitalet, University of Oslo, Norway.

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis

|June 1, 1995

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Activated platelets and microvesicles express factor Xa activity on their surfaces. This intrinsic factor Xa (FXa) contributes to thrombin generation, highlighting a novel procoagulant mechanism in platelet activation.

Area of Science:

Hematology
Biochemistry
Cell Biology

Background:

Activated platelets and microvesicles possess procoagulant properties.
Stimulated platelets expose negatively charged phospholipids and factor Va on their surfaces.

Purpose of the Study:

To investigate if activated platelets and microvesicles express intrinsic factor Xa (FXa) activity.
To determine if FXa originates from external sources or is expressed on platelet surfaces.

Main Methods:

Flow cytometry using anti-FXa monoclonal antibody.
Chromogenic substrate assays for FXa and thrombin.
Prothrombinase assay with factor X-depleted plasma.
Platelet activation using calcium ionophore or thrombin receptor agonist peptide.

Related Experiment Videos

Main Results:

Flow cytometry confirmed FXa presence on activated platelet surfaces.
Platelet-derived microvesicles and activated platelets exhibited intrinsic FXa activity.
Thrombin generation was inhibited by FXa-specific inhibitors (rTAP, antithrombin III/heparin) and leupeptin.

Conclusions:

Activated platelets and their derived microvesicles express functional FXa on their surfaces.
This intrinsic FXa contributes to the procoagulant activity of activated platelets.
Platelets represent a significant source of intrinsic FXa, impacting thrombin generation.