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[Denitrification by Bacillus licheniformis]

F Pichinoty, J L Garcia, C Job

    Canadian Journal of Microbiology
    |January 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

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    This study assessed Bacillus licheniformis denitrification, finding limited nitrogen gas production. Most strains reduced nitrate to nitrite but struggled with further reduction to N2O or N2.

    Area of Science:

    • Microbiology
    • Environmental Science
    • Biochemistry

    Background:

    • Denitrification is a crucial microbial process in nitrogen cycling.
    • Bacillus licheniformis is a common bacterium with potential roles in nitrogen metabolism.
    • Understanding denitrification pathways in bacteria is vital for environmental applications.

    Purpose of the Study:

    • To evaluate the denitrifying capacity of fifteen Bacillus licheniformis strains.
    • To investigate the specific reduction pathways and intermediates involved in denitrification by these strains.
    • To determine the enzymatic activities related to denitrification.

    Main Methods:

    • Culturing of Bacillus licheniformis strains under anaerobic conditions with various nitrogen compounds.
    • Gas chromatography analysis of gaseous products (N2, N2O) from cultures.

    Related Experiment Videos

  • Enzyme assays for nitrate reductase and nitrite reductase activities in cell extracts.

    • Main Results:

    • Most Bacillus licheniformis strains showed slow and irregular N2 production from nitrate.
    • Three strains did not produce any detectable gas.
    • Strains efficiently reduced nitrate to nitrite but showed limited N2O or N2 production; nitric oxide was reduced to N2O.
    • Nitrate reductase A was synthesized anaerobically, but nitrite reductase activity was negligible.

    Conclusions:

    • Bacillus licheniformis strains possess limited denitrification capabilities, primarily reducing nitrate to nitrite.
    • The key enzymes for complete denitrification (nitrite reductase) appear to be largely inactive or absent in these strains.
    • Further research is needed to explore the environmental implications of these findings.