A GOD electrode free from the influence of ethanol

The influence of ethanol on GOD electrode was investigated. When enzyme electrode was made by the Sandwich method, 0.1% (V/V) ethanol had an obvious influence on the response of the electrode with 4.3% deviation. The influence increased with increasing ethanol content of the samples. This influence can be avoided by using a nylon mesh GOD electrode, which was successfully used for the determination of glucose in the range of 5-20 mM with ethanol content up to 9% (V/V). Such an electrode showed good reproducibility, stable response activity and long storage life ( > 30 days).