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Error-tolerant protein database searching using peptide product-ion spectra
Rapid Communications in Mass Spectrometry : RCM
|January 1, 1995
Summary
This study introduces a fast protein identification method using peptide mass data. It accurately identifies proteins in samples, even with modifications like derivatization or post-translational changes.
Area of Science:
- Proteomics
- Biochemistry
- Analytical Chemistry
Background:
- Protein identification is crucial for biological research.
- Existing methods can be challenged by sample modifications.
Purpose of the Study:
- To develop a rapid and robust method for protein identification.
- To enable accurate protein database matching despite experimental data anomalies.
Main Methods:
- Utilizes peptide product ion mass data for analysis.
- Employs a novel matching algorithm for database comparison.
Main Results:
- Demonstrates high speed of analysis.
- Achieves reliable protein identification even with derivatization or post-translational modifications.
Conclusions:
- The described method offers a powerful tool for protein identification in complex samples.
- Enhances the ability to analyze proteomes with inherent sample variations.