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Updated: Aug 17, 2026

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
Published on: December 26, 2012
Changes in beta 1- and beta 2-adrenoceptor mRNA levels in alveolar type II cells during cultivation
1Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Abstract:
Alveolar type II cells, the progenitor of type I epithelial cells, synthesize and secrete pulmonary surfactant. In this study, we determined the levels of mRNA for beta 1- and beta 2-adrenoceptor in cultured type II cells using a quantitative RNase protection assay. The mRNA levels for beta 1- and beta 2-adrenoceptors in the freshly isolated cells were 0.51 +/- 0.07 and 1.92 +/- 0.19 amol/microgram total cellular RNA, respectively. In the cells cultured for over 2 days, only beta 1-adrenoceptor mRNA level was remarkably reduced. These results suggest that each mRNA expression may be separately regulated in cultured alveolar type II cells.
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