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Related Experiment Video

Updated: Jul 12, 2026

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging
12:15

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Published on: October 3, 2017

[Isolated enterocytes as an object of functional morphology]

A M Ugolev, A Iu Tsibulevskiĭ, A I Mamatakhunov

    Morfologiia (Saint Petersburg, Russia)
    |January 1, 1995
    PubMed
    Summary

    Isolated rat jejunum enterocytes maintain their characteristics and function. Their mitochondria and succinate dehydrogenase activity are influenced by the cell

    Area of Science:

    • Cell Biology
    • Histology
    • Biochemistry

    Background:

    • Isolated enterocytes are crucial for studying intestinal function.
    • Understanding enterocyte morphology and cytochemistry is vital for digestive physiology research.

    Purpose of the Study:

    • To characterize isolated rat jejunum enterocytes morphologically and cytochemically.
    • To investigate the functional state of isolated enterocytes based on their metabolic activity and mitochondrial behavior.

    Main Methods:

    • Mechanical isolation of rat jejunum enterocytes after ethylenediaminetetraacetic acid disodium salt treatment.
    • Staining with hematoxylin, carmine, and Janus green.
    • Histochemical reactions for alkaline phosphatase, succinate dehydrogenase, lactate dehydrogenase, ATP-ase, and glycosaminoglycans.

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  • Incubation of enterocytes with various substrates (glucose, maltose, glycine, triolein) under different oxygenation conditions.

    • Main Results:

    • Isolated enterocytes exhibited significant resemblance to in-vivo intestinal epithelium in cytological and cytochemical features.
    • Histochemical analysis revealed the presence of key enzymes and glycosaminoglycans.
    • Mitochondrial topography and succinate dehydrogenase activity were found to be dependent on the functional state of the enterocytes, influenced by substrate availability and oxygenation.

    Conclusions:

    • The isolation method preserves the essential characteristics of rat jejunum enterocytes.
    • Enterocyte functional state, particularly mitochondrial activity, is adaptable and responsive to metabolic and environmental cues.