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Corneal epithelial fluorescein staining

G Wilson1, H Ren, J Laurent

  • 1School of Optometry, University of Alabama at Birmingham 35294-4390, USA.

Journal of the American Optometric Association
|July 1, 1995
PubMed
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Fluorescein staining of the corneal epithelium is due to individual cells taking up the dye, not intercellular spaces or cell dropout. This finding clarifies interpretations of corneal staining patterns.

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Microscopy

Background:

  • Discrepancies exist regarding fluorescein staining of the corneal epithelial surface.
  • Theories include cell dropout with pooling, intercellular space filling, or direct cell staining.

Purpose of the Study:

  • To investigate the precise mechanism of fluorescein staining on the corneal epithelial surface.
  • To resolve conflicting interpretations in the existing literature.

Main Methods:

  • Rabbit corneas were stained with fluorescein.
  • Examination was performed using biomicroscopy and epifluorescent microscopy after excision.

Main Results:

  • Fluorescein staining was definitively attributed to individual epithelial cells.

Related Experiment Videos

  • No evidence supported fluorescein pooling in cell dropout areas or filling of intercellular spaces.
  • Micropunctate staining was identified as optimal cell staining, termed hyperfluorescence.

    • Conclusions:

    • Fluorescein directly stains corneal epithelial cells, even with abrasion.
    • This understanding allows for a revised interpretation of various staining phenomena, such as "salt and pepper" staining.