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Sug1 modulates yeast transcription activation by Cdc68
Q Xu1, R A Singer, G C Johnston
1Department of Microbiology, Dalhousie University, Halifax, Nova Scotia, Canada.
Molecular and Cellular Biology
|November 1, 1995
Summary
The SUG1 gene suppresses defects in the Cdc68 protein, which is essential for gene transcription in yeast. Sug1
Area of Science:
- Molecular biology
- Yeast genetics
- Transcription regulation
Background:
- Cdc68 protein is crucial for gene transcription in Saccharomyces cerevisiae.
- Understanding proteins interacting with Cdc68 is key to elucidating transcriptional regulation.
- Previous studies implicated the SUG1 gene in transcriptional regulation and proteolysis.
Purpose of the Study:
- To molecularly characterize mutations in the SUG1 gene as suppressors of cdc68-1.
- To investigate the mechanism by which SUG1 affects Cdc68 activity.
- To determine the role of Sug1 in transcriptional regulation.
Main Methods:
- Genetic screening for suppressor mutations of the cdc68-1 temperature-sensitive allele.
- Molecular characterization of the identified SUG1 suppressor gene.
- Analysis of yeast phenotypes, including temperature sensitivity and lethality.
- Investigation of protein stability and transcriptional activity in vivo.
Main Results:
- Mutations in SUG1 reversed most phenotypes associated with the cdc68-1 mutation.
- Sug1 suppression did not rescue the lethality of a cdc68 null allele, indicating restoration of Cdc68 activity.
- Sug1 appears to antagonize Cdc68's activator function, repressing transcription in vivo.
- Altered Sug1 activity modulated mutant Cdc68 activity, suggesting a regulatory interaction.
Conclusions:
- The SUG1 gene plays a significant role in regulating Cdc68 protein activity.
- Sug1 acts antagonistically to Cdc68's role in transcriptional activation.
- Sug1 functions as a transcriptional repressor in yeast.