Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mutation frequency in human blood cells increases with age

M Akiyama1, S Kyoizumi, Y Hirai

  • 1Department of Radiobiology, Radiation Effects Research Foundation, Hiroshima, Japan.

Mutation Research
|October 1, 1995
PubMed
Summary

Mutant cell frequencies in human blood increase with age. This study measured mutations in T lymphocytes and erythrocytes, finding a significant age-dependent rise in mutant frequencies for all genes studied.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pituitary adenylate cyclase-activating polypeptide produces a phase shift associated with induction of mPer expression in the mouse suprachiasmatic nucleus.

Neuroscience·2002
Same author

Formation of cornified cell envelope in human hair follicle development.

The British journal of dermatology·2002
Same author

Detection of chlorophyll d' and pheophytin a in a chlorophyll d-dominating oxygenic photosynthetic prokaryote Acaryochloris marina.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2002
Same author

Atrioventricular nodal re-entrant tachycardia with two functionally discrete fast pathways.

The Journal of international medical research·2002
Same author

Effects of metal combinations on cytotoxicity evaluation using a dynamic extraction method.

Dental materials journal·2002
Same author

Telomerase activity in peripheral blood mononuclear cells from patients with SLE.

Annals of the rheumatic diseases·2002

Area of Science:

  • Human genetics
  • Molecular biology
  • Aging research

Background:

  • Assessing genetic mutations in peripheral blood cells is crucial for understanding aging and disease.
  • The frequency of mutant cells can be measured using techniques like colony formation assays and flow cytometry.
  • Previous studies have suggested a link between aging and increased genetic instability.

Purpose of the Study:

  • To investigate the age-dependent changes in mutant cell frequencies in human peripheral blood.
  • To analyze mutations in specific genes within T lymphocytes (hypoxanthine-guanine phosphoribosyltransferase and T-cell receptor) and erythrocytes (glycophorin A).
  • To explore potential mechanisms underlying the observed age-related increases in mutant frequencies.

Main Methods:

  • Utilized colony formation assays and flow cytometry to quantify rare mutant lymphocytes and erythrocytes.

Related Experiment Videos

  • Analyzed blood samples from several hundred individuals across a wide age range (0-96 years).
  • Focused on mutant frequencies of the hypoxanthine-guanine phosphoribosyltransferase and T-cell receptor genes in T lymphocytes, and the glycophorin A gene in erythrocytes.

    • Main Results:

    • A significant increase in the mutant frequency of all investigated genes was observed with advancing age.
    • The frequency of glycophorin A mutants in erythrocytes showed a clear age-dependent accumulation.
    • T-cell mutant frequencies also increased with age, suggesting a complex regulatory mechanism.

    Conclusions:

    • Mutant cell frequencies in both T lymphocytes and erythrocytes significantly increase with age in humans.
    • The accumulation of mutations in hematopoietic stem cells over time is a likely explanation for age-related increases in glycophorin A mutants.
    • The age-dependent rise in T-cell mutations may involve a balance between mutation generation and cellular loss.