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Updated: Jul 31, 2026

Dual Somatic Recordings from Gonadotropin-Releasing Hormone (GnRH) Neurons Identified by Green Fluorescent Protein (GFP) in Hypothalamic Slices
Published on: February 24, 2010
[Specific hypothalamic proteins]
Abstract:
We had previously shown the presence of four protein fractions in hypothalamus of cattle having a coronarodilatatory effect. In this study we have developed methods for the isolation of these proteins and have studied some of their physicochemical properties, amino acid composition, molecular weight, iso-electric point etc. The results obtained have shown that the methods used enable the isolation of quite pure coronaroactive proteins, the content of which in the hypothalamus is equal to 12 mg/kg of fresh tissue. A study of the amino acid composition of these proteins has shown that they are not of the same type. They also differ in molecular weight, in their electrophoretic properties on 7.5% polyacrylamide gel and their iso-electric points. The possibility of a dual function of these proteins as carriers and precursors of corresponding neurohormones is discussed.
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