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Updated: Jul 31, 2026

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
Published on: March 10, 2010
[Neurospecific protein-hormonal complexes]
1Institute of Biochemistry named after G.Kh. Buniatian of NAN of Armenia, Yerevan.
Abstract:
New cardioactive protein-hormone complexes (PHC) were identified, and then separated and purified in magnocellular foci of hypothalamus of oxen and some other animals. It was proved that the PHC are specific for nervous tissue, their subsell localisation was studied, concentration and distribution in an organism were identified. It was discovered, that under dissociation of PHC, its high-molecular "apoforms" represent new specific glicoproteins, and the low-molecular combinations related to them in a non-covalent way are glicopeptides with clear cardioactive properties inherent in the respective native PHC. The above glicoproteins are transport molecules for the cardioactive neurohormones of hypothalamus, and are the basis for mediation of the inter-neurone interaction and the finction of intracellular connection. In addition, it was proved that these glicoproteins can exist in the form of preprohormone forms of cardiotropic neurohormones of hypothalamus: "K", "S", and "G".
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