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Mobilization of Bacillus thuringiensis plasmid pTX14-3

L Andrup1, H H Bendixen, G B Jensen

  • 1National Institute of Occupational Health, Department of Toxicology and Biology, Copenhagen, Denmark.

Plasmid
|May 1, 1995
PubMed
Summary

The Bacillus thuringiensis subsp. israelensis (Bti) plasmid pTX14-3

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Bacillus thuringiensis subsp. israelensis (Bti) harbors the plasmid pTX14-3.
  • The plasmid pTX14-3 contains a gene, mob14-3, homologous to mobilization proteins in other gram-positive bacteria.
  • Bti exhibits an aggregation-mediated conjugation system for DNA transfer.

Purpose of the Study:

  • To investigate the mobilization kinetics of Bti plasmid pTX14-3 derivatives.
  • To assess the role of the mob14-3 gene in plasmid mobilization and stability.
  • To analyze the impact of a DNA locus suppressing high-molecular-weight DNA formation on transfer frequency.

Main Methods:

  • Utilized the Bti aggregation-mediated conjugation system.
  • Compared mobilization kinetics of modified pTX14-3 plasmid derivatives.
  • Analyzed the effect of deleting a specific DNA fragment and the presence of the mob14-3 gene.

Main Results:

  • Deleting a DNA fragment suppressing high-molecular-weight DNA formation doubled plasmid transfer frequency.
  • The mob14-3 gene did not enhance plasmid mobilization frequency.
  • The mob14-3 gene appeared to increase plasmid stability during exponential growth.

Conclusions:

  • The identified DNA locus significantly influences pTX14-3 transfer frequency.
  • The mob14-3 gene's primary role may be plasmid stabilization rather than mobilization.
  • Further research is needed to fully elucidate the function of mob14-3 in Bti.

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