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Introduction of Pseudomonas aeruginosa mutator phage D3112 into Alcaligenes eutrophus strain CH34
V Krylov1, C Merlin, A Toussaint
1Institute for Genetics of Microorganisms, Moscow.
Abstract:
We have investigated the possibility of growing mutator phages from Pseudomonas aeruginosa on various isolates of Alcaligenes eutrophus. Although none out of 10 A. eutrophus strains were susceptible to infection with any of the phages tested, phage D3112 could be readily transferred in our model strain CH34 by means of an RP4::D3112 plasmid. CH34/RP4::D3112 lysogens were stable and produced phages. However, neither mitomycin C nor UV treatment increased the phage yield.
Insights
Researchers explored growing Pseudomonas aeruginosa mutator phages on Alcaligenes eutrophus. While direct infection failed, phage D3112 was successfully transferred via a plasmid, forming stable lysogens that produced phages without induction.
Area of Science:
- Microbiology
- Bacteriology
- Virology
Background:
- Pseudomonas aeruginosa phages are potential tools for bacterial control.
- Alcaligenes eutrophus is a related bacterial species.
- Investigating phage-host interactions is crucial for phage therapy development.
Purpose of the Study:
- To determine if mutator phages from P. aeruginosa can infect and propagate on A. eutrophus isolates.
- To explore alternative methods for transferring phages between these species.
Main Methods:
- Testing susceptibility of 10 A. eutrophus strains to various phages.
- Transferring phage D3112 using a constructed RP4::D3112 plasmid in a model strain (CH34).
- Assessing the stability and phage production of resulting lysogens.
- Evaluating the effect of mitomycin C and UV induction on phage yield.
Main Results:
- No A. eutrophus strains were susceptible to direct phage infection.
- Phage D3112 was successfully transferred to the model strain CH34 via the RP4::D3112 plasmid.
- The resulting CH34/RP4::D3112 lysogens were stable and continuously produced phages.
- Neither mitomycin C nor UV treatment enhanced phage yield from the lysogens.
Conclusions:
- Direct infection of A. eutrophus by P. aeruginosa mutator phages is not feasible.
- Plasmid-mediated transfer is an effective method for establishing phage lysogeny in new hosts.
- Lysogens formed through this method are stable but do not exhibit inducible phage production via standard methods.