Inheritance of allozyme loci in Bombina: one linkage group established

Two hybridizing European species of fire-bellied toads, Bombina bombina and B. variegata, have alternate electromorphs fixed at a number of allozyme loci. Segregation of alleles at seven allozyme loci (Ldh-1, Mdh-1, Ak, Ck, Gpi, Np, and Est-beta) was studied in a backross progeny of an F1 interspecific hybrid male and a B. bombina female. Mendelian inheritance of allozyme forms at all seven loci was ascertained. Except for two loci, Gpi and Est-beta, which were found to be tightly linked (1 cM apart), other loci showed independent segregation.