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Impaired tumor phenotypes in class II major histocompatibility complex antigen-inducible cells originated from human

S Kawamoto1, Y Inoue, Y Shinozaki

  • 1Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka, Japan.

Biochemical and Biophysical Research Communications
|October 4, 1995
PubMed

The class II major histocompatibility complex (MHC) antigens play important roles in T cell activation and are thought to be involved in tumor development. Using anti-class II antibodies with interferon gamma (IFN-gamma), we have here selected class II MHC antigen positive cancer cells from the human lung adenocarcinoma cell line A549, which is originally negative (> 95%) for the antigens. A part of the class II antigen-positive cells presented a flat morphology, which was not observed in the parental A549 cells. Class II antigen expression in these flat cells was IFN-gamma inducible; there was a correlation between the inducibility and phenotypic changes. A class II antigen-inducible flat subline restored the ability of contact inhibition and anchorage-dependent growth as well as lost tumorigenicity in athymic mice.

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