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Updated: Jul 1, 2026

Determining the Phagocytic Activity of Clinical Antibody Samples
Published on: November 30, 2011
The mode of actions of glyceraldehyde-3-phosphate dehydrogenase identified as an immunoglobulin production
T Sugahara1, S Shirahata, T Sasaki
1Department of Biotechnology, College of Agriculture, Ehime University, Japan.
Abstract:
Immunoglobulin production stimulating factor-II alpha, which enhances immunoglobulin production of human and mouse hybridomas was purified from cell lysate of human Burkitt's lymphoma, Namalwa cells, and identified as glyceraldehyde-3-phosphate dehydrogenase. The enhancement of immunoglobulin production with this enzyme was not linked with its enzymatic activity. The enzyme enhanced immunoglobulin productivity of transcription-suppressed hybridomas, but did not enhance that of translation-suppressed hybridomas. From these results, it is suggested that this enzyme takes part in the post-translational control or the enhancement of translation activity to stimulate immunoglobulin production of hybridomas.
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