Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Preliminary three-dimensional model for nematode thick filament core

H F Epstein1, G Y Lu, P R Deitiker

  • 1Department of Neurology, Baylor College of Medicine, Houston, Texas 77030, USA.

Journal of Structural Biology
|September 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Prevalence and influencing factors of chronic kidney disease-associated pruritus in patients undergoing maintenance hemodialysis].

Zhonghua yi xue za zhi·2026
Same author

[Study on predicting new onset heart failure events in patients with hypertrophic cardiomyopathy using machine learning algorithms based on clinical and magnetic resonance features].

Zhonghua xin xue guan bing za zhi·2024
Same author

[Application value of neutrophil lymphocyte ratio combined with age in the diagnosis of dual-phenotype hepatocellular carcinoma].

Zhonghua yi xue za zhi·2022
Same author

[Time to initial diagnosis of imported malaria and its influencing factors in Jiangsu Province].

Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control·2022
Same author

Association with HLA DQ of early onset myasthenia gravis in Southeast Texas region of the United States.

International journal of immunogenetics·2010
Same author

Genetic analysis of myosin II assembly and organization in model organisms.

Cellular and molecular life sciences : CMLS·2005
Same journal

Ultrastructural evidence of autophagy-related processes and mitochondrial remodeling in the myxozoan parasite Henneguya piaractus.

Journal of structural biology·2026
Same journal

Architecture and dynamics of a supramolecular oxygen transport system in human homogentisate 1,2-Dioxygenase.

Journal of structural biology·2026
Same journal

Connecting pathways between mineralized fibrocartilage and bone at the Achilles tendon insertion.

Journal of structural biology·2026
Same journal

Structural and functional characterization of thermostable EstS1 esterase for BHET degradation.

Journal of structural biology·2026
Same journal

Following the white rabbit: multiscale 2D3D correlative imaging of bone structure.

Journal of structural biology·2026
Same journal

The mantis shrimp eye imaged in 3D using 4th generation synchrotron multiscale phase contrast tomography.

Journal of structural biology·2026
See all related articles

Researchers developed a 3D model of thick filament cores from C. elegans, revealing paramyosin subfilaments and inner tubules. This structure is key to understanding muscle assembly and regulation.

Area of Science:

  • Muscle Biology
  • Structural Biology
  • Biophysics

Background:

  • Thick filament structure and assembly are fundamental to muscle function.
  • Paramyosin is a major structural protein in invertebrate muscle thick filaments.

Purpose of the Study:

  • To elucidate the three-dimensional structure of thick filament cores.
  • To propose a model for the assembly mechanism of thick filaments.

Main Methods:

  • Isolation of thick filament cores from Caenorhabditis elegans.
  • Electron microscopy (negative staining and cryo-EM).
  • Image analysis including Fourier transforms, 3D reconstruction, and molecular modeling.

Main Results:

  • A preliminary 3D model of thick filament cores was generated.

Related Experiment Videos

  • Paramyosin forms an outer sheath of seven subfilaments around inner tubules.
  • A specific molecular arrangement with staggered paramyosin molecules and cross-linking tubules was identified.

    • Conclusions:

    • The proposed model explains the structural organization of thick filament cores.
    • Identified interaction sites suggest critical roles for paramyosin and inner tubular proteins in filament assembly and regulation.