[Classical and pulsed gel electrophoresis of biopolymers. Theory and applications]

The work is devoted to the modern theory of gel electrophoresis in stationary and alternating electric fields, and it brings together results of numerous theoretical and experimental studies hitherto conducted. Main attention is drawn to the approximation definitions used in theory and to cases of divergences between theory and experimentation. Basic methods of gel electrophoresis and electrotransfer are principally analysed that appeared not long ago and showed to be greatly perspective. The resolution ability, advantages and disadvantages of pulsed methods are analysed, methods and instrumentation used in laboratory practice are described. Quantitative assessment of most important dependencies of applied significance are shown.