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Monoclonal antibodies against Vi antigen production and characterization

D Pieńkowska, D Kunikowska, R Głośnicka

    Bulletin of the Institute of Maritime and Tropical Medicine in Gdynia
    |January 1, 1993
    PubMed

    As a result of fusion of in vitro immunized mouse splenocytes and myeloma Sp2/0 cells, stable hybridomas secreting monoclonal antibodies against Vi antigen were obtained. The monoclonal antibodies were of IgM class. Reactivity of monoclonal antibodies to Vi antigen of different origin was tested. The reactivity was found to be independent of O-acetylation degree of Vi antigen.

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