Related Experiment Videos
Use of vectorette and subvectorette PCR to isolate transgene flanking DNA
M J Allen1, A Collick, A J Jeffreys
1Department of Genetics, University of Leicester, UK.
PCR Methods and Applications
|October 1, 1994
Abstract:
Vectorette PCR permits the specific amplification of DNA segments flanking a known DNA sequence. It enables the application of the PCR where sequence information is only available for one primer site. We now show that vectorette PCR can be used for the systematic mapping and retrieval of transgene flanking DNA. We also show that the sequence of large vectorette PCR fragments can be obtained without cloning, by the production of subvectorette fragments.