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Related Experiment Videos

Pseudorabies virus mutants as transneuronal markers

J M Sams1, A S Jansen, T C Mettenleiter

  • 1Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Brain Research
|July 31, 1995
PubMed
Summary

Researchers compared four pseudorabies virus (PRV) strains for transneuronal labeling in rat ocular sympathetic pathways. Bartha beta-gal demonstrated the highest specificity, making it a valuable tool for neural circuit mapping.

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Area of Science:

  • Neuroscience
  • Virology
  • Genetics

Background:

  • Pseudorabies virus (PRV) is utilized for transneuronal tracing in neural circuits.
  • Genetically engineered PRV strains offer modified properties for specific research applications.
  • Understanding viral properties is crucial for effective neural pathway analysis.

Purpose of the Study:

  • To evaluate the transneuronal labeling capabilities of three genetically modified Bartha PRV strains against the parental strain.
  • To determine the optimal infectious dose for specific transneuronal labeling of sympathetic preganglionic neurons.
  • To assess the specificity and infectivity rates of different PRV mutants in the ocular sympathetic pathway.

Main Methods:

  • Transneuronal labeling was assessed in the ocular sympathetic pathway of rats.

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  • Five microliters of viral stocks (Bartha PRV, Bartha beta-gal, Bartha gIIIKa, Bartha gI+) were injected into the anterior chamber of the eye.
  • Labeling patterns were analyzed 4 days post-injection to determine optimal infectious doses and specificity.

    • Main Results:

    • An inverse relationship was observed between viral specificity and infectivity rate.
    • Bartha beta-gal exhibited the highest specific labeling (76%), while Bartha gI+ showed the lowest (10%).
    • Bartha gIIIKa labeled more second-order and third-order neurons compared to Bartha beta-gal and Bartha PRV.

    Conclusions:

    • Bartha beta-gal is a highly specific virus for transneuronal labeling studies in the ocular sympathetic pathway.
    • Bartha gI+ is not suitable for transneuronal labeling due to its low specificity.
    • Modified PRV strains, particularly Bartha beta-gal, offer enhanced utility for mapping neural circuits.