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Morphological changes induced in HEp-2 cells by Salmonella typhimurium porins

L De Martino1, C Nazzaro, S Concilio

  • 1Institute of Microbiology, Faculty of Medicine, University of Naples, Italy.

Journal of Submicroscopic Cytology and Pathology
|October 1, 1995
PubMed

Insights

Salmonella typhimurium porins affect human laryngeal cells by altering cytoskeleton and nuclear membranes. These findings reveal porins

Area of Science:

  • Microbiology
  • Cell Biology
  • Toxicology

Background:

  • Salmonella typhimurium is a pathogen known to cause foodborne illness.
  • Bacterial porins are outer membrane proteins that can mediate interactions with host cells.
  • The cellular effects of S. typhimurium porins on human epithelial cells require further elucidation.

Purpose of the Study:

  • To investigate the impact of Salmonella typhimurium porins on human laryngeal epithelial (HEp-2) cells.
  • To characterize the cellular and ultrastructural changes induced by varying doses of porins.

Main Methods:

  • Exposure of HEp-2 cells to different doses of S. typhimurium porins.
  • Analysis using fluorescence microscopy to observe microfilament organization.
  • Examination via transmission electron microscopy for ultrastructural modifications.

Main Results:

  • Alterations in microfilament organization within HEp-2 cells were observed, suggesting cytoskeleton involvement.
  • Ultrastructural analysis revealed significant membrane modifications in the cells.
  • These modifications extended to the nuclear membrane, indicating a broader cellular impact.

Conclusions:

  • Salmonella typhimurium porins induce significant morphological changes in human laryngeal epithelial cells.
  • The cell cytoskeleton plays a role in the cellular response to porin exposure.
  • Porins can cause ultrastructural damage to cellular and nuclear membranes.

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