Melatonin modifies prolactin release induced by opiate antagonists in male rats

The effect of melatonin on the prolactin (PRL) release induced by treatment with naloxone, naloxone methyliodide, naltrexone and nalmefene were studied in adult male rats. Subcutaneous melatonin injection (1.4 mg/Kg) had no significant effect on serum PRL levels, but decreased by 29% and 26% respectively the inhibitory effect potency of naltrexone (2.5 mg/Kg) and nalmefene (2 mg/Kg) on PRL secretion after simultaneous injections. The inhibitory effect potency of naloxone on PRL release increased (16%) when it was administered with melatonin. Simultaneous injection of melatonin with naloxone methyliodide (2.8 mg/Kg) inhibited PRL release (77.5%) while naloxone methyliodide alone did not modify this secretion. The results obtained with a quaternary opioid antagonist indicate that the opioid receptor type which mediates PRL response is located inside the blood-brain barrier. Our findings show that opiate antagonists and their quaternary ammonium salts affect secretion of PRL through mechanism susceptible to the influence of melatonin.