Effective blotting of ultrathin polyacrylamide gels anchored to a solid matrix

J Heukeshoven1, R Dernick

  • 1Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Germany.

Electrophoresis
|May 1, 1995
PubMed

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SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...