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Functional expression of the human CHIP28 water channel in a yeast secretory mutant
V Laizé1, G Rousselet, J M Verbavatz
1Département de Biologie Cellulaire et Moléculaire, Service de Biologie Cellulaire, CEA-Saclay, Gif sur Yvette, France.
FEBS Letters
|October 16, 1995
Abstract:
The temperature-sensitive Saccharomyces cerevisiae mutant strain NY17, deficient in the secretory pathway (sec6-4 mutation), is used for the heterologous expression of the human CHIP28 water channel. After a heat-shock, the protein is present in partially purified post-golgi secretory vesicles. Immunodetection and water transport studies, directly made on the vesicles, showed that CHIP28 is highly expressed and active in the yeast membranes.