Related Experiment Videos
Construction of a recombinant cellulolytic Escherichia coli
R Srivastava1, G P Kumar, K K Srivastava
1Division of Microbial Genetics, Central Drug Research Institute, Lucknow, India.
Gene
|October 16, 1995
Abstract:
A 1.2-kb DNA fragment from Bacillus subtilis CD4 encoding endo-beta-1,4-glucanase and cellobiase activities was cloned and expressed in Escherichia coli. Carboxymethylcellulase (CMCase) and cellobiase activities were detected in a cell-free lysate of recombinant (re-) E. coli (Ec). The re-enzymes were functional in Ec, as it utilized carboxymethylcellulose, soluble cellulose and cellobiose as sole carbon sources for growth.