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[Microdetermination of fluoride in human bones (author's transl)]
Clinica Chimica Acta; International Journal of Clinical Chemistry
|January 15, 1979
Summary
A new spectrophotometric method accurately determines fluoride in human bones using cerium(III)-alizarin complexan and dimethylsulfoxide. This rapid technique analyzes fluoride after microdiffusion without requiring sample mineralization.
Area of Science:
- Analytical Chemistry
- Biochemistry
Context:
- Fluoride analysis in biological samples is crucial for understanding bone health and disease.
- Existing methods for bone fluoride determination can be time-consuming or require extensive sample preparation.
Purpose:
- To develop and describe a novel, rapid, and selective spectrophotometric method for quantifying fluoride in human bone tissue.
- To establish a microdiffusion technique for isolating hydrofluoric acid from bone samples prior to analysis.
Summary:
- A spectrophotometric method utilizing cerium(III)-alizarin complexan in 25% dimethylsulfoxide was optimized for fluoride determination.
- Fluoride was separated from human bone samples via microdiffusion as hydrofluoric acid in Petri dishes, eliminating the need for mineralization.
- The method demonstrates selectivity, accuracy, and speed for bone fluoride analysis.
Impact:
- Provides a valuable analytical tool for researchers and clinicians studying bone metabolism and fluoride toxicity.
- Offers a simplified and efficient alternative to traditional methods for bone fluoride measurement.
- Facilitates more accessible and widespread fluoride assessment in human bone samples.