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Related Concept Videos

Voltage-gated Ion Channels01:26

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Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
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The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
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Related Experiment Video

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Basophil Activation Test for Allergy Diagnosis
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Voltage-dependent ion channels on human basophils: do they exist?

F Beauvais1, C Burtin, J Benveniste

  • 1INSERM U200, Université Paris-Sud, Clamart, France.

Immunology Letters
|May 1, 1995
PubMed
Summary

Human basophils may possess voltage-dependent calcium channels. Experiments show high potassium solutions trigger calcium-dependent histamine release in these cells, suggesting a role in allergic responses.

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Area of Science:

  • Immunology
  • Cell Biology
  • Physiology

Background:

  • The presence of voltage-dependent ion channels, specifically calcium (Ca2+) channels, on non-excitable cells like human basophils is debated.
  • While Ca2+ influx alone doesn't trigger basophil secretion, it enhances it, indicating a role for calcium signaling.

Purpose of the Study:

  • To investigate the presence and function of voltage-dependent Ca2+ pathways in human basophils.
  • To determine if membrane depolarization can influence Ca2+-dependent histamine release in these cells.

Main Methods:

  • Utilized a two-signal model for stimulating human basophils.
  • Applied phorbol 12-myristate 13-acetate (PMA) as the first signal.
  • Used high potassium (K+) solutions to induce membrane depolarization as a second signal, assessing Ca2+-dependent histamine release.

Main Results:

  • Phorbol 12-myristate 13-acetate (PMA)-treated human basophils exhibited Ca2+-dependent histamine release when exposed to a depolarizing high K+ solution.
  • High K+ solution alone did not induce histamine release, indicating a requirement for prior cell activation.

Conclusions:

  • The findings suggest the existence of voltage-dependent structures linked to Ca2+ pathways in human basophils.
  • This voltage-dependent Ca2+ influx may contribute to the enhanced histamine release observed in activated basophils, potentially playing a role in allergic reactions.